| Data | In casa | Ospite | Risultato |
|---|---|---|---|
| 29/11/24 20.45 | Harrogate Town FC | Gainsborough Trinity | 1:0 |
| 30/11/24 12.30 | Wealdstone FC | Wycombe Wanderers | 0:2 |
| 30/11/24 16.00 | FC Walsall | Charlton Athletic | 0:4 |
| 30/11/24 16.00 | Salford City | Cheltenham Town | 2:0 |
| 30/11/24 16.00 | Accrington Stanley | Swindon Town | 6:3d.c.r |
| 30/11/24 16.00 | FC Barnsley | Bristol Rovers | 3:4d.c.r |
| 30/11/24 16.00 | Leyton Orient | Oldham Athletic | 2:1d.t.s |
| 30/11/24 16.00 | Exeter City | FC Chesterfield | 2:0 |
| 30/11/24 16.00 | FC Morecambe | Bradford City | 1:0 |
| 30/11/24 16.00 | Stockport County | Brackley Town | 3:1 |
| 30/11/24 16.00 | Peterborough United | Notts County | 4:3 |
| 30/11/24 16.00 | Stevenage FC | Mansfield Town | 0:1 |
| 30/11/24 16.00 | Cambridge United | Wigan Athletic | 1:2d.t.s |
| 30/11/24 16.00 | Crawley Town | Lincoln City | 3:4 |
| 30/11/24 20.15 | AFC Wimbledon | Dagenham and Redbridge | 1:2 |
| 01/12/24 13.00 | Kettering Town FC | Doncaster Rovers | 1:2d.t.s |
| 01/12/24 14.00 | FC Blackpool | Birmingham City | 1:2 |
| 01/12/24 15.00 | Burton Albion | FC Tamworth | 4:5d.c.r |
| 01/12/24 15.00 | FC Reading | Harborough Town FC | 5:3d.t.s |
| 01/12/24 16.15 | Solihull Moors FC | Bromley FC | 1:2 |